The Rho-kinase inhibitor H-1152P suppresses the wound-healing activities of human Tenon's capsule fibroblasts in vitro.

@article{Tura2007TheRI,
  title={The Rho-kinase inhibitor H-1152P suppresses the wound-healing activities of human Tenon's capsule fibroblasts in vitro.},
  author={Ayseguel Tura and Salvatore Grisanti and Katrin Petermeier and Sigrid Henke-Fahle},
  journal={Investigative ophthalmology & visual science},
  year={2007},
  volume={48 5},
  pages={2152-61}
}
PURPOSE To analyze the role of Rho-kinase signaling in the wound-healing activities of human Tenon's capsule fibroblasts by using H-1152P, a potent inhibitor of this kinase, in vitro. METHODS The optimal concentration of H-1152P was determined by MTT test. Cell proliferation was measured by BrdU incorporation and Ki-67 immunostaining, whereas cell viability was investigated by ethidium homodimer-1 dye exclusion. The actin cytoskeleton organization was demonstrated by alpha-smooth muscle actin… CONTINUE READING