The Resolution Revolution

@article{Khlbrandt2014TheRR,
  title={The Resolution Revolution},
  author={Werner K{\"u}hlbrandt},
  journal={Science},
  year={2014},
  volume={343},
  pages={1443 - 1444}
}
Advances in detector technology and image processing are yielding high-resolution electron cryo-microscopy structures of biomolecules. [Also see Report by Amunts et al.] Precise knowledge of the structure of macromolecules in the cell is essential for understanding how they function. Structures of large macromolecules can now be obtained at near-atomic resolution by averaging thousands of electron microscope images recorded before radiation damage accumulates. This is what Amunts et al. have… 

Visualization of biological macromolecules at near-atomic resolution: cryo-electron microscopy comes of age.

  • A. Mitra
  • Chemistry
    Acta crystallographica. Section F, Structural biology communications
  • 2019
TLDR
Atomic resolution single-particle analysis, without the need for crystals, now promises to resolve problems in structural biology that were intractable just a few years ago.

High-End Data Collection for Single-Particle Cryo-EM

  • F. Weis
  • Chemistry
    Microscopy and Microanalysis
  • 2019
Single-particle cryogenic transmission electron microscopy (cryo-EM) can be used to elucidate the 3D structure of macromolecular complexes. The sample is embedded in a thin layer of vitreous ice and

Cryo-electron microscopy reaches atomic resolution.

TLDR
Cryo­EM is a decades-old structural­biology technique that has garnered increasing interest since around 2013 due to a series of techno­ logical and algorithmic advances that together drove a striking improvement in the resolution obtainable by this technique (described as the ‘resolution revolution’).

Biological Applications at the Cutting Edge of Cryo-Electron Microscopy

TLDR
Developments in sample preparation methods and substrates, detectors, phase plates, and cryo-correlative light and electron microscopy that have contributed to this expansion are reviewed.

Recent Advances in Single Particle Cryo-electron Microscopy and Cryo-electron Tomography to Determine the Structures of Biological Macromolecules

  • M. Dutta
  • Chemistry
    Journal of the Indian Institute of Science
  • 2018
A detailed three-dimensional structure of macromolecular assemblies is necessary to understand their function which in turn helps to understand life. Cryo-electron microscopy (cryo-EM) is a powerful

Cryo-electron Microscopy Analysis of Structurally Heterogeneous Macromolecular Complexes

  • S. Jonić
  • Chemistry
    Computational and structural biotechnology journal
  • 2016

Atomic-resolution protein structure determination by cryo-EM.

TLDR
A 1.25 Å-resolution structure of apoferritin is reported with a newly developed electron microscope that provides, to the authors' knowledge, unprecedented structural detail and a substantial improvement in the quality of the cryo-EM density map.

Cryo-EM is a powerful tool, but helical applications can have pitfalls.

TLDR
The enormous potential for using cryo-EM, and also the pitfalls possible for helical assemblies when a near-atomic level of resolution is not reached are highlighted.

Cryo-Electron Tomography

TLDR
An overview of recent advances in sample preparation, data acquisition and data processing is provided, including technology for focused ion beam milling, correlative light and electron microscopy, phase-plate imaging and direct electron detection, which show that these developments can be used synergistically to generate 3-D images of cells of unprecedented quality.

How cryo-EM is revolutionizing structural biology.

...

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