The Relevance of the Urinary Concentration of Ephedrines in Anti-Doping Analysis: Determination of Pseudoephedrine, Cathine, and Ephedrine After Administration of Over-the-Counter Medicaments

@article{StranoRossi2009TheRO,
  title={The Relevance of the Urinary Concentration of Ephedrines in Anti-Doping Analysis: Determination of Pseudoephedrine, Cathine, and Ephedrine After Administration of Over-the-Counter Medicaments},
  author={Sabina Strano-Rossi and Daniele Leone and Xavier de la Torre and Francesco Botr{\`e}},
  journal={Therapeutic Drug Monitoring},
  year={2009},
  volume={31},
  pages={520-526}
}
This article describes a method for the detection and quantitation of cathine, pseudoephedrine, ephedrine, and methylephedrine in urine, using their deuterated analogues as internal standards and derivatization to form the corresponding trimethylsilyl derivatives after a simple liquid-liquid extraction. The study was designed to evaluate whether the urinary cutoff values set by the World Anti-Doping Agency for the banned ephedrines (cathine >5 μg/mL, ephedrine and methylephedrine >10 μg/mL) can… 
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References

SHOWING 1-8 OF 8 REFERENCES
Metabolites of ephedrines in human urine after administration of a single therapeutic dose.
TLDR
Exretion studies of the ephedrine-related drugs have been performed to better understand the metabolic yields of ephedrines in urine and found that a single clinical dose of EPH may exceed threshold level in sport drug testing if the urine samples are tested within approximately 8h post-administration.
Elimination of ephedrines in urine following multiple dosing: the consequences for athletes, in relation to doping control.
TLDR
Following therapeutic, multiple dosing, drug levels remain above the IOC cut-off levels for a minimum of 6 h and 16 h following final doses of phenylpropanolamine and pseudoephedrine, respectively.
Urinary excretion of chlorpheniramine and pseudoephedrine in humans.
TLDR
In spite of expected changes in its biological half-life, the overall amount of unchanged pseudoephedrine excreted in urine was not affected by urine pH, presumably because it is primarily excreting in urine as intact drug.
Ephedrines in over-the-counter cold medicines and urine specimens collected during sport competitions.
TLDR
It is likely that the high incidence of doping violations for ephedrine-related substances was related to misuse of ephedrines present in most OTC common cold medicines and some dietary supplements for relieving cold symptoms, reducing body weight, and preserving muscle.
Clinical Pharmacokinetics of Amfetamine and Related Substances
TLDR
Because of the physicochemical properties of amfetamine-type stimulants, this group of drugs is one of the most suitable for drug testing in non-conventional matrices and has recently gained much attention because of its possible applications in clinical and forensic toxicology.
Investigation of the silylation of ephedrines using N-methyl-N-trimethylsilyl-trifluoroacetamide.
  • G. Forsdahl, G. Gmeiner
  • Chemistry, Medicine
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
  • 2004
TLDR
The gas chromatography--mass spectrometry method was found to be well suited for quantification of ephedrines in doping control.
Pharmacology of ephedra alkaloids and caffeine after single‐dose dietary supplement use
TLDR
This work assessed the pharmacokinetics and pharmacodynamics of a dietary supplement that contains ma huang (Ephedra) and guarana (caffeine) and concluded that these herbal stimulants are safe and effective as dietary supplements.
Clinical pharmacokinetics of amphetamine and relates substances
  • Clin Pharmacokinet
  • 2004