The Region between Transmembrane Domains 1 and 2 of the Reduced Folate Carrier Forms Part of the Substrate-binding Pocket*

@article{Flintoff2003TheRB,
  title={The Region between Transmembrane Domains 1 and 2 of the Reduced Folate Carrier Forms Part of the Substrate-binding Pocket*},
  author={Wayne F. Flintoff and Frederick M. R. Williams and Heather Sadlish},
  journal={Journal of Biological Chemistry},
  year={2003},
  volume={278},
  pages={40867 - 40876}
}
A functional cysteine-less form of the hamster reduced folate carrier protein was generated by alanine replacement of the 14 cysteine residues. The predicted 12-transmembrane topology was examined by replacing selected amino acids, predicted to be exposed to the extracellular or cytosolic environments, with cysteines. The location of these cysteines was defined by their accessibility to biotin maleimide in the presence or absence of specific blocking agents. Amino acids predicted to be exposed… 

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