The Redox State of Cytochrome C Modulates Resistance to Methotrexate in Human MCF7 Breast Cancer Cells

@article{Barros2013TheRS,
  title={The Redox State of Cytochrome C Modulates Resistance to Methotrexate in Human MCF7 Breast Cancer Cells},
  author={Susana Raquel Moura Barros and N{\'u}ria Mencia and Laura Rodr{\'i}guez and Carlota Oleaga and Conceiç{\~a}o Santos and V{\'e}ronique No{\'e} and Carlos J. Ciudad},
  journal={PLoS ONE},
  year={2013},
  volume={8}
}
Background Methotrexate is a chemotherapeutic agent used to treat a variety of cancers. However, the occurrence of resistance limits its effectiveness. Cytochrome c in its reduced state is less capable of triggering the apoptotic cascade. Thus, we set up to study the relationship among redox state of cytochrome c, apoptosis and the development of resistance to methotrexate in MCF7 human breast cancer cells. Results Cell incubation with cytochrome c-reducing agents, such as… Expand
Effect of Paullinia cupana on MCF-7 breast cancer cell response to chemotherapeutic drugs.
TLDR
Guarana improved the antiproliferative effect of chemotherapeutic agents, causing a decrease of >40% in cell growth after 72 h of exposure and an interaction of guarana with the chemotherAPEutic drugs, which requires confirmation by in vivo complementary studies. Expand
Regulation of cytochrome c in respiration, apoptosis, neurodegeneration and cancer: The good, the bad and the ugly
TLDR
The regulation and multiple functions of mammalian Cytc are reviewed, including respiration, reactive oxygen species (ROS) scavenging under healthy conditions, ROS production via p66, and cardiolipin oxidation during apoptosis, and a proposed targeting of signaling cascades as a therapeutic avenue in conditions including neurodegeneration and cancer are proposed. Expand
Evaluation of oxidative stress via protein expression of glutathione S-transferase and cytochrome p450 (CYP450) ısoenzymes in psoriasis vulgaris patients treated with methotrexate
TLDR
A significant increase in the tissue levels of, either expression of GST, or CYP, which has important role in drug metabolism and oxidative stress is found, which may play a major role in the psoriasis pathogenesis. Expand
Biochemical and transcriptional analyses of cadmium-induced mitochondrial dysfunction and oxidative stress in human osteoblasts
TLDR
Cd induced mitochondrial dysfunction which appeared to be associated with oxidative stress in human osteoblasts, and the activity of enzymes involved in mitochondrial respiration, membrane potential, and mitochondrial morphology were analyzed. Expand
Altered Protein S-Glutathionylation Identifies a Potential Mechanism of Resistance to Acetaminophen-Induced Hepatotoxicity
TLDR
It is found that S-glutathionylation of the rate-limiting glutathione-synthesizing enzyme, glutamate cysteine ligase, was markedly increased in Gstp1/2−/− mice in response to APAP. Expand
Correlation between the potency of flavonoids for cytochrome c reduction and inhibition of cardiolipin‐induced peroxidase activity
TLDR
Flavonoids that behave as Cyt c reductants also inhibited the pro-apoptotic CL-induced peroxidase activity of CyT c, indicating that modulation of Cytochrome c signaling are probable mechanisms behind the protective biological activities of flavonoids. Expand
Methotrexate-F127 conjugated mesoporous zinc hydroxyapatite as an efficient drug delivery system for overcoming chemotherapy resistance in osteosarcoma cells.
TLDR
Mesoporous zinc-substituted hydroxyapatite has been synthesized and served as a drug delivery vehicle owing to its biocompatibility and high drug loading capacity and MTX-F127@ZnHAP nanoparticles are pH-responsive nanocarriers with precise controlled drug release and targeting effect. Expand
Hypomethylation of the GSTM1 promoter is associated with ovarian endometriosis.
TLDR
New evidence is provided to support the notion that endometriosis may be an epigenetic disease and that the over-expression of GSTM1 could significantly increase viability and inhibit apoptosis in endometrial epithelial cells following hormone treatment and withdrawal. Expand
Stability and immunogenicity properties of the gene-silencing polypurine reverse Hoogsteen hairpins.
TLDR
Stability experiments revealed that PPRHs, unlike siRNAs, do not activate the innate inflammatory response, and a new type of molecule to decrease gene expression named polypurine reverse Hoogsteen hairpins that bind to polypyrimidine targets in the DNA was developed. Expand
Polypurine reverse Hoogsteen hairpins as a gene therapy tool: in vitro development and in vivo validation
TLDR
A comparative study to explore the in vitro efficacy in terms of cell viability and apoptosis of different types of PPRHs -either Coding or Template-, against different target regions -intron, exon or promoter- in different cancer cell lines and found that two P PRHs against the “survivin” promoter were the most effective and their mechanism of action was investigated. Expand
...
1
2
...

References

SHOWING 1-10 OF 75 REFERENCES
Glutathione in Cancer Biology and Therapy
TLDR
An analysis of links among GSH, adaptive responses to stress, molecular mechanisms of invasive cancer cell survival and death, and sensitization of metastatic cells to therapy shows that acceleration of GSH efflux facilitates selective GSH depletion in metastasis cells. Expand
UDP-glucuronosyltransferase 1A6 overexpression in breast cancer cells resistant to methotrexate.
TLDR
Cells incubated with anticancer drugs susceptible to glucuronidation, such as tamoxifen or irinotecan, together with methotrexate, showed a lesser degree of cytotoxicity, due to UGT1A6 induction, and the pharmacological effect of this induction should be taken into account when combining metotrexate with other drugs that are glucuronidated. Expand
Role of cellular glutathione and glutathione S-transferase in the expression of alkylating agent cytotoxicity in human breast cancer cells.
TLDR
GSH affects the cytotoxicity of different AAs to different extents, basal GST expression in MCF-7 cells does not play a major role in AA metabolism, and depletion of cellular GSH by pretreatment with BCNU or cyclophosphamide may correspond to a useful strategy for enhancing the anti-tumor activity of other AAs given in a sequential combination. Expand
The role of mitochondria in bee venom-induced apoptosis in human breast cancer MCF7 cells.
TLDR
It is demonstrated that BV induced apoptosis via a mitochondria-dependent pathway based on the changes of delta psi m, AIF and EndoG release in MCF7 cells. Expand
Glutathione and glutathione-dependent enzymes in cancer drug resistance.
  • L. McLellan, C. Wolf
  • Biology, Medicine
  • Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy
  • 1999
TLDR
New data demonstrating the importance of glutathione pathways in cytoprotection and greater understanding of the mechanisms which regulate their function reveal a number of new targets for novel anti-cancer agents. Expand
Ornithine decarboxylase prevents methotrexate-induced apoptosis by reducing intracellular reactive oxygen species production
TLDR
It is found MTX could induce caspase-dependent apoptosis and promote ROS generation together with disrupting the mitochondrial membrane potential of HL-60 and Jurkat T cells and demonstrate that MTX-induced apoptosis is ROS-dependent and occurs along a mitochondria-mediated pathway. Expand
Regulation of apoptosis by the redox state of cytochrome c.
TLDR
Regulation of the redox state of cytochrome c potentially enables regulation of the intrinsic pathway of apoptosis at a relatively late stage. Expand
Glucose Metabolism Inhibits Apoptosis in Neurons and Cancer Cells by Redox Inactivation of Cytochrome c
TLDR
The results uncover a striking similarity in apoptosis regulation between neurons and cancer cells and provide insight into an adaptive advantage offered by the Warburg effect for cancer cell evasion of apoptosis and for long-term neuronal survival. Expand
Suppression of the pro-apoptotic function of cytochrome c by singlet oxygen via a haem redox state-independent mechanism.
TLDR
The data suggest that singlet oxygen eliminates the pro-apoptotic ability of oxidized cyt c not via the reduction of haem, but via the modification of amino acid residues that are required for apoptosome formation. Expand
Overexpression of S100A4 in human cancer cell lines resistant to methotrexate
TLDR
β-catenin overexpression experiments support a possible involvement of the Wnt signaling pathway in S100A4 transcriptional regulation in HT29 cells and highlight a role for S 100A4 in MTX resistance. Expand
...
1
2
3
4
5
...