The Reaction Mechanism of the Mitochondrial Pyridine Nucleotide Transhydrogenase

@inproceedings{ChenS2001TheRM,
  title={The Reaction Mechanism of the Mitochondrial Pyridine Nucleotide Transhydrogenase},
  author={Shiuan ChenS and Philippines Richard and Guilloryt},
  year={2001}
}
The addition of a purified mitochondrial pyridine nucleotide transhydrogenase enzyme preparation to complex I (NADH-CoQ reductase) results in a significant increase in the NADPH-AcPyAD' transhydrogenase activity of the complex without influencing the NADH-AcPyAD+ transhydrogenase activity. When subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the presence of complex I, the purified transhydrogenase enzyme preparation was found to co-migrate with the Mr = 130,000 (130K… CONTINUE READING