The Rat IgGFcγBP and Muc2 C-Terminal Domains and TFF3 in Two Intestinal Mucus Layers Bind Together by Covalent Interaction

@inproceedings{Yu2011TheRI,
  title={The Rat IgGFcγBP and Muc2 C-Terminal Domains and TFF3 in Two Intestinal Mucus Layers Bind Together by Covalent Interaction},
  author={Hao Yu and Yonghong He and Xin Zhang and Zhihong Peng and Yongtao Yang and Rong Zhu and Jianying Bai and Yin Tian and Xiaohuan Li and Wensheng Chen and Dianchun Fang and Rongquan Wang},
  booktitle={PloS one},
  year={2011}
}
BACKGROUND The secreted proteins from goblet cells compose the intestinal mucus. The aims of this study were to determine how they exist in two intestinal mucus layers. METHODOLOGY/PRINCIPAL FINDINGS The intestinal mucosa was fixed with Carnoy solution and immunostained. Mucus from the loose layer, the firm layer was gently suctioned or scraped, respectively, lysed in SDS sample buffer with or without DTT, then subjected to the western blotting of rTFF3, rIgGFcγBP or rMuc2. The non-reduced or… CONTINUE READING
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