The Ras mutant D119N is both dominant negative and activated.

  title={The Ras mutant D119N is both dominant negative and activated.},
  author={Robbert H. Cool and Gunter Nils Schmidt and Christian U Lenzen and Heino Prinz and Dieter Vogt and Alfred Wittinghofer},
  journal={Molecular and cellular biology},
  volume={19 9},
The introduction of mutation D119N (or its homolog) in the NKxD nucleotide binding motif of various Ras-like proteins produces constitutively activated or dominant-negative effects, depending on the system and assay. Here we show that Ras(D119N) has an inhibitory effect at a cell-specific concentration in PC12 and NIH 3T3 cells. Biochemical data strongly suggest that the predominant effect of mutation D119N in Ras-a strong decrease in nucleotide affinity-enables this mutant (i) to sequester its… CONTINUE READING

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