The RNase protection assay.

Abstract

The RNase protection assay is a sensitive method for transcription start-site localization. It begins with an RNA probe that is uniformly labeled by incorporation of one [α-(32)P]NTP, usually [α-(32)P]UTP. The RNA probe is synthesized by bacteriophage RNA polymerase (SP6, T7, or T3), which initiates transcription from specific phage promoters that have been… (More)
DOI: 10.1101/pdb.prot071910

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Cite this paper

@article{Carey2013TheRP, title={The RNase protection assay.}, author={Michael Carey and Craig Peterson and Stephen Smale}, journal={Cold Spring Harbor protocols}, year={2013}, volume={2013 3} }