The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme.

@article{GuerrierTakada1983TheRM,
  title={The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme.},
  author={Cecilia Guerrier-Takada and Ken Gardiner and Terry L. Marsh and Norman R. Pace and S. Altman},
  journal={Cell},
  year={1983},
  volume={35 3 Pt 2},
  pages={849-57}
}
The RNA moieties of ribonuclease P purified from both E. coli (M1 RNA) and B. subtilis (P-RNA) can cleave tRNA precursor molecules in buffers containing either 60 mM Mg2+ or 10 mM Mg2+ plus 1 mM spermidine. The RNA acts as a true catalyst under these conditions whereas the protein moieties of the enzymes alone show no catalytic activity. However, in buffers containing 5-10 mM Mg2+ (in the absence of spermidine) both kinds of subunits are required for enzymatic activity, as shown previously. In… CONTINUE READING
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