The RNA-mediated silencing of one of the Pin genes in allohexaploid wheat simultaneously decreases the expression of the other, and increases grain hardness.

@article{Gasparis2011TheRS,
  title={The RNA-mediated silencing of one of the Pin genes in allohexaploid wheat simultaneously decreases the expression of the other, and increases grain hardness.},
  author={Sebastian Gasparis and Waclaw Orczyk and Wojciech Zalewski and Anna Nadolska-Orczyk},
  journal={Journal of experimental botany},
  year={2011},
  volume={62 11},
  pages={4025-36}
}
The RNAi-mediated silencing of Pina and Pinb, the two genes responsible for the grain texture of allohexaploid wheat, was induced and analysed in two wheat cultivars, Kontesa and Torka. A characterization of the two genes in non-transgenic plants revealed that Pinb carries a point mutation, designated Pinb-D1c in both cultivars. This mutation does not influence transcript abundance or protein content. Two silencing cassettes of the hpRNA type were constructed and used for stable transformation… CONTINUE READING
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