The RNA component of human telomerase

@article{Feng1995TheRC,
  title={The RNA component of human telomerase},
  author={Jia Feng and W D Funk and SS Wang and S. S. Weinrich and AA Avilion and C. Chiu and RR Adams and Edwin Chang and RC Allsopp and J K Yu and Et Al.},
  journal={Science},
  year={1995},
  volume={269},
  pages={1236 - 1241}
}
Eukaryotic chromosomes are capped with repetitive telomere sequences that protect the ends from damage and rearrangements. Telomere repeats are synthesized by telomerase, a ribonucleic acid (RNA)-protein complex. Here, the cloning of the RNA component of human telomerase, termed hTR, is described. The template region of hTR encompasses 11 nucleotides (5'-CUAACCCUAAC) complementary to the human telomere sequence (TTAGGG)n. Germline tissues and tumor cell lines expressed more hTR than normal… 
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
The currently known components of the telomerase complex are described and an update is provided on the molecular mechanisms of human telomere regulation to provide an update in antiaging and anticancer therapy.
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TLDR
The current understanding of telomerase in breast cancer development is outlined and potential utilities in diagnosis, prognosis, and therapy are critically evaluated.
Regulation of Telomerase by DNA and Protein Interactions
TLDR
It was found that human TEN interacted with singlestranded telomeric DNA and restored function, in trans, to an hTERT mutant lacking hTEN, suggesting that dimerization may occur via TEN-TERT interactions.
Telomerase RNA is more than a DNA template
TLDR
The Stem Terminus Element (STE) is distant from the templating core of telomerase in both the linear and RNA secondary structure, but, nonetheless, affects the fidelity of telomere sequence addition and, in turn, the ability oftelomere binding proteins to bind and protect chromosome ends.
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References

SHOWING 1-10 OF 44 REFERENCES
Functional characterization and developmental regulation of mouse telomerase RNA
TLDR
The shorter template regions of the mouse and other rodent telomerase RNAs could account for the shorter distribution of products (processivity) generated by the mouse enzyme relative to the human telomersase.
Telomerase activity in vivo in human malignant hematopoietic cells.
TLDR
The data demonstrate that telomerase activation in vivo seems to be a common event in B cell neoplasias with a mature immunophenotype like non-Hodgkin's lymphoma and myeloma, in contrast to acute leukemias of B, T or myeloid cell origin.
Functional evidence for an RNA template in telomerase.
TLDR
Functional analysis showed that the sequence 5' CAAAACCCCAAA 3' in this RNA is the template for synthesis of telomeric TTTTGGGG repeats by the Euplotes telomerase, and Telomerase can now be defined as a specialized reverse transcriptase.
TLC1: template RNA component of Saccharomyces cerevisiae telomerase.
TLDR
The discovery of TLC1 confirms the existence of telomerase in S. cerevisiae and may facilitate both the analysis of this enzyme and an understanding of telomere structure and function.
Telomeres shorten during ageing of human fibroblasts
TLDR
The amount and length of telomeric DNA in human fibroblasts does in fact decrease as a function of serial passage during ageing in vitro and possibly in vivo.
Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity.
TLDR
The results suggest that chromosomes with short (TTAGGG)n tracts are recombinogenic, critically shortened telomeres may be incompatible with cell proliferation and stabilization of telomere length by telomerase may be required for immortalization.
Stabilization of short telomeres and telomerase activity accompany immortalization of Epstein-Barr virus-transformed human B lymphocytes
TLDR
It is proposed that telomerase activity is required for immortality, and detected this enzyme in a variety of immortal human cells transformed by different viruses, indicating that Telomerase activation may be a common step in immortalization.
Telomerase activity in human ovarian carcinoma.
TLDR
It is reported that extremely short telomeres are maintained in metastatic cells of epithelial ovarian carcinoma and that tumor cells, but not isogenic nonmalignant cells, express telomerase, suggesting that progression of malignancy is ultimately dependent upon activation of telomersase and that telomerases inhibitors may be effective antitumor drugs.
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