The RNA World on Ice: A New Scenario for the Emergence of RNA Information

@article{Vlassov2005TheRW,
  title={The RNA World on Ice: A New Scenario for the Emergence of RNA Information},
  author={Alexander V Vlassov and Sergei A. Kazakov and Brian H. Johnston and Laura F. Landweber},
  journal={Journal of Molecular Evolution},
  year={2005},
  volume={61},
  pages={264-273}
}
The RNA world hypothesis refers to a hypothetical era prior to coded peptide synthesis, where RNA was the major structural, genetic, and catalytic agent. Though it is a widely accepted scenario, a number of vexing difficulties remain. In this review we focus on a missing link of the RNA world hypothesis—primitive miniribozymes, in particular ligases, and discuss the role of these molecules in the evolution of RNA size and complexity. We argue that prebiotic conditions associated with freezing… 

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References

SHOWING 1-10 OF 93 REFERENCES

Boundaries for an RNA world.

  • M. Yarus
  • Biology
    Current opinion in chemical biology
  • 1999

Ligation activity of fragmented ribozymes in frozen solution: implications for the RNA world.

It is reported that truncated and fragmented derivatives of the hairpin ribozyme can catalyze ligation of a wide variety of RNA molecules to a given sequence in frozen solution despite having little or no activity under standard solution conditions.

Ribozymes--why so many, why so few?

  • F. Schmidt
  • Chemistry, Biology
    Molecules and cells
  • 1999
It is proposed that the efficiency of some small ribozymes may have been augmented by other RNAs acting as transactivators, leading to a question of whether they could have been synthesized by a primitive replicator.

In search of an RNA replicase ribozyme.

Experimental RNA evolution.

  • Landweber
  • Biology
    Trends in ecology & evolution
  • 1999

The origins of RNA catalysis in ribozymes.

  • D. Lilley
  • Biology, Chemistry
    Trends in biochemical sciences
  • 2003

The antiquity of RNA-based evolution

It is concluded that DNA- and protein-based life was preceded by a simpler life form based primarily on RNA, referred to as the 'RNA world', during which the genetic information resided in the sequence of RNA molecules and the phenotype derived from the catalytic properties of RNA.

Peptide synthesis through evolution

RNAs can facilitate peptide bond formation by providing a template for activated amino acids to react and can catalyze a variety of functions that would have been necessary in a pre-protein world.

RNA-Catalyzed RNA Polymerization: Accurate and General RNA-Templated Primer Extension

An RNA molecule is described that catalyzes the type of polymerization needed for RNA replication, which uses nucleoside triphosphates and the coding information of an RNA template to extend an RNA primer by the successive addition of up to 14 nucleotides.

RIBOZYME ENGINEERING AND EARLY EVOLUTION

In this article, the accomplishments and implications of ribozyme engineering are reviewed and critiqued.
...