The RIN: an RNA integrity number for assigning integrity values to RNA measurements

  title={The RIN: an RNA integrity number for assigning integrity values to RNA measurements},
  author={Andreas Schroeder and Odilo M. Mueller and Susanne Stocker and Ruediger Salowsky and Michael M Leiber and Marcus Gassmann and Samar Lightfoot and Wolfram Menzel and Martin Granzow and Thomas Ragg},
  journal={BMC Molecular Biology},
  pages={3 - 3}
The integrity of RNA molecules is of paramount importance for experiments that try to reflect the snapshot of gene expression at the moment of RNA extraction. Until recently, there has been no reliable standard for estimating the integrity of RNA samples and the ratio of 28S:18S ribosomal RNA, the common measure for this purpose, has been shown to be inconsistent. The advent of microcapillary electrophoretic RNA separation provides the basis for an automated high-throughput approach, in order… CONTINUE READING
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