The RFC clamp loader: structure and function.

  title={The RFC clamp loader: structure and function.},
  author={Nina Y. Yao and Mike O'Donnell},
  journal={Sub-cellular biochemistry},
The eukaryotic RFC clamp loader couples the energy of ATP hydrolysis to open and close the circular PCNA sliding clamp onto primed sites for use by DNA polymerases and repair factors. Structural studies reveal clamp loaders to be heteropentamers. Each subunit contains a region of homology to AAA+ proteins that defines two domains. The AAA+ domains form a right-handed spiral upon binding ATP. This spiral arrangement generates a DNA binding site within the center of RFC. DNA enters the central… CONTINUE READING