The RBCC gene RFP2 (Leu5) encodes a novel transmembrane E3 ubiquitin ligase involved in ERAD.

  title={The RBCC gene RFP2 (Leu5) encodes a novel transmembrane E3 ubiquitin ligase involved in ERAD.},
  author={Mikael S. Lerner and M. Corcoran and Diana A Cepeda and Michael L Nielsen and Roman A. Zubarev and Fredrik Ponten and Mathias Uhl{\'e}n and Sophia Hober and Dan Grand{\'e}r and Olle Sangfelt},
  journal={Molecular biology of the cell},
  volume={18 5},
RFP2, a gene frequently lost in various malignancies, encodes a protein with RING finger, B-box, and coiled-coil domains that belongs to the RBCC/TRIM family of proteins. Here we demonstrate that Rfp2 is an unstable protein with auto-polyubiquitination activity in vivo and in vitro, implying that Rfp2 acts as a RING E3 ubiquitin ligase. Consequently, Rfp2 ubiquitin ligase activity is dependent on an intact RING domain, as RING deficient mutants fail to drive polyubiquitination in vitro and are… CONTINUE READING


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