The Pseudomonas oleovorans alkane hydroxylase gene. Sequence and expression.

@article{Kok1989ThePO,
  title={The Pseudomonas oleovorans alkane hydroxylase gene. Sequence and expression.},
  author={Menno Kok and Roelof Oldenhuis and M P Van Der Linden and P Raatjes and Jaap Kingma and P H van Lelyveld and Bernard Witholt},
  journal={The Journal of biological chemistry},
  year={1989},
  volume={264 10},
  pages={5435-41}
}
We have identified and sequenced the Pseudomonas OCT plasmid-encoded alkane hydroxylase gene (alkB) and its promoter. The transcription initiation site of the alkBAC mRNA was determined by nuclease S1 mapping. A putative interaction site with RNA-polymerase was identified based on homology of the alk promoter with other Pseudomonas promoters. The alkB gene encodes a 401-amino acid polypeptide which, despite an unusual codon composition, can be expressed at high levels in Escherichia coli and… CONTINUE READING