The Protein Protocols Handbook

@inproceedings{Walker2002ThePP,
  title={The Protein Protocols Handbook},
  author={JOHN M. Walker},
  booktitle={Humana Press},
  year={2002}
}

Figures and Tables from this paper

Hemagglutinating activity of proteins from Parkia speciosa seeds
Proteins from Parkia speciosa Hassk. (Fabaceae) seeds were extracted and stepwise precipitated using ammonium sulfate. Proteins precipitated with 25% ammonium sulfate were separated by affinityExpand
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The binding of key fishy off-flavor compounds to silver carp proteins: a thermodynamic analysis
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Biochemical Characterization of a Novel Monospecific Endo-β-1,4-Glucanase Belonging to GH Family 5 From a Rhizosphere Metagenomic Library
TLDR
The cellulolytic enzyme CelRH5 is a novel monospecific endo-β-1,4-glucanase belonging to the glycosyl hydrolase family 5 (GH5), which is psychrotolerant and retains activity in the presence of final cellulose degradation products, metal ions and various reagents, which are common in many technological processes. Expand
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TLDR
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Silencing cathepsin L expression reduces Myzus persicae protein content and the nutritional value as prey for Coccinella septempunctata
TLDR
It is shown that expression of cathepsin L, which encodes a cysteine protease that functions in aphid guts, can be reduced by expression of an RNAi construct in transgenic tobacco. Expand
Bioaugmentation of the anaerobic digestion of food waste by dungs of herbivore, carnivore, and omnivore zoo animals
TLDR
The results indicate that bioaugmentation with zoo animals dung is worthy of further investigation as a strategy for improving the biomethane recovery from organic wastes. Expand
Evaluating analytical methods for the characterization of lipids, proteins and carbohydrates in organic substrates for anaerobic co-digestion.
TLDR
In general, lipid-rich samples yielded more biogas than samples rich in proteins and carbohydrates, however, sample rich in protein and carbohydrates had faster biogAS production rates. Expand
Silencing cathepsin L expression reduces Myzus persicae protein content and the nutritional value as prey for Coccinella septempunctata
TLDR
It is shown that expression of cathepsin L, a cysteine protease that functions in aphid guts, can be reduced by expression of an RNAi construct in transgenic tobacco. Expand
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References

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A rapid, sensitive method has been developed to detect antibody-antigen complexes on "Western blots" by using Tween 20 to separate and blot the antigens onto nitrocellulose. Expand
Direct Blue 71 staining of proteins bound to blotting membranes
TLDR
A sensitive staining method for protein blots using Direct Blue 71 is described, which is based on the selective binding of dye molecules to proteins in acidic solution and produces bluish violet colored bands and may be more practical than other dye‐based stains or metal-based stains for routine laboratory purposes. Expand
A luminescent europium complex for the sensitive detection of proteins and nucleic acids immobilized on membrane supports.
TLDR
The reversible nature of the protein staining procedure allows for subsequent biochemical analyses, such as immunoblotting, lectin staining, and mass spectrometry. Expand
Pyrogallol red‐molybdate: A reversible, metal chelate stain for detection of proteins immobilized on membrane supports
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Certain metal complexes selectively interact with proteins immobilized on solid‐phase membrane supports to form brightly colored products, and the triazine dye‐ferrous complexes and the ferrocyanide‐ferric complexes provide the most sensitive detection of proteins immobilization on membranes. Expand
Staining methods in gel electrophoresis, including the use of multiple detection methods.
TLDR
The present review highlights the utilization of combined protein-labeling and -staining methodologies in gel electrophoresis including selected applications in polyacrylamide gels and solid membrane matrixes. Expand
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TLDR
Metal chelate stains were determined to be fully compatible with immunoblotting, N-terminal, and in situ internal protein microsequencing and were of equivalent sensitivity to colloidal gold stain. Expand
A high-affinity protein stain for western blots, tissue prints, and electrophoretic gels.
TLDR
The method for protein staining using copper phthalocyanine 3,4',4'',4'''-tetrasulfonic acid tetrasodium salt is described, which is more sensitive than any previously described dye-based nonspecific proteinStaining system and easily reversible. Expand
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Additional members of the rat liver lamin polypeptide family. Structural and immunological characterization.
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TLDR
Two-dimensional tryptic peptide mapping and Western blotting with affinity-purified chicken and human sera have been utilized to examine the structural relationships and the tissue distribution of these quantitatively less prominent mammalian lamins, suggesting that levels of lamins D and E are regulated independently from those of the major lamins. Expand
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