The Predicted Coiled-coil Domain of Myosin 10 Forms a Novel Elongated Domain That Lengthens the Head*

  title={The Predicted Coiled-coil Domain of Myosin 10 Forms a Novel Elongated Domain That Lengthens the Head*},
  author={Peter J. Knight and Kavitha Thirumurugan and Yuhui Xu and Fei Wang and Arnout P. Kalverda and Walter F. Stafford and James R. Sellers and Michelle Peckham},
  journal={Journal of Biological Chemistry},
  pages={34702 - 34708}
Myosin 10 contains a region of predicted coiled coil 120 residues long. However, the highly charged nature and pattern of charges in the proximal 36 residues appear incompatible with coiled-coil formation. Circular dichroism, NMR, and analytical ultracentrifugation show that a synthesized peptide containing this region forms a stable single α-helix (SAH) domain in solution and does not dimerize to form a coiled coil even at millimolar concentrations. Additionally, electron microscopy of a… 

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