The PrP-like protein Doppel binds copper.

  title={The PrP-like protein Doppel binds copper.},
  author={Kefeng Qin and Janaky Coomaraswamy and Peter Mastrangelo and Ying Yang and Stan Lugowski and Chris Petromilli and Stanley B Prusiner and Paul E. Fraser and J. M. Goldberg and Avijit Chakrabartty and David Westaway},
  journal={The Journal of biological chemistry},
  volume={278 11},
Doppel (Dpl) is a glycosylphosphatidylinositol-anchored protein expressed in the testis. It exhibits 26% sequence identity with the prion protein (PrP) but lacks the octarepeat region implicated as the major copper-binding domain. Contrary to expectations, Cu(II) induced a 26% reduction in the intrinsic fluorescence of Dpl(27-154) and a calculated K(d) for a single-site model of 0.16 +/- 0.08 microm. Other metals had minimal effects on fluorescence quenching. Matrix-assisted laser desorption… CONTINUE READING

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