The PomXYZ Proteins Self-Organize on the Bacterial Nucleoid to Stimulate Cell Division.

  title={The PomXYZ Proteins Self-Organize on the Bacterial Nucleoid to Stimulate Cell Division.},
  author={Dominik Schumacher and Silke Bergeler and Andrea Harms and Janet Vonck and Sabrina Huneke-Vogt and Erwin Frey and Lotte S{\o}gaard-Andersen},
  journal={Developmental cell},
  volume={41 3},

Figures from this paper

Regulation of Pom cluster dynamics in Myxococcus xanthus

A one-dimensional model that includes the nucleoid, the Pom cluster and PomZ proteins is investigated and it is found that a mechanism based on the diffusive PomZ fluxes on the nucleoids into the cluster can explain the latter’s midnucleoid localization for a broad parameter range.

A phase-separated biomolecular condensate nucleates polymerization of the tubulin homolog FtsZ to spatiotemporally regulate bacterial cell division

It is demonstrated that PomY forms liquid-like biomolecular condensates by phase separation, while PomX self-assembles into filaments generating a single large cellular structure, supporting this mechanism’s ancient origin.

PomX, a ParA/MinD ATPase activating protein, is a triple regulator of cell division in Myxococcus xanthus

It is demonstrated that the architecturally diverse ATPase activating proteins of ParA/MinD ATPases are highly modular and use the same mechanism to activate their cognate ATPase via a short positively charged N-terminal extension.

Can a flux-based mechanism explain positioning of protein clusters in a three-dimensional cell geometry?

A mathematical model is introduced that accounts for the three-dimensional shape of the nucleoid, such that nucleoid-bound PomZ dimers can diffuse past the cluster without interacting with it, and concludes that a flux-based mechanism allows for cluster positioning in a biologically realisticThree-dimensional cell geometry.

Bacterial cell division at a glance

This Cell Science at a Glance article provides an overview of the initial stages of cytokinetic ring assembly, dynamics and activation during bacterial cell division.

The MinDE system is a generic spatial cue for membrane protein distribution in vitro

In vitro reconstitution is used to show that MinDE oscillations also regulate unrelated membrane proteins spatiotemporally into patterns and gradients by forming a moving physical barrier.

Biochemistry of the key spatial regulators MipZ and PopZ in Caulobacter crescentus

The inhibitory mode of action of the polar element MipZ on FtsZ polymerization is uncovered and the interaction regions of Mip z are identified, which reveal that the DNAand ParB-binding regions are overlapping and mainly constituted of positively charged residues, whereas two distinct regions appear to be involved in Ftsz-binding.

Regulation of Cell Polarity in Motility and Cell Division in Myxococcus xanthus.

This review covers recent findings concerning the spatiotemporal regulation of motility and cell division in M. xanthus and illustrates how the study of diverse bacteria may uncover novel mechanisms involved in regulating bacterial cell polarity.

The E. coli MinCDE system in the regulation of protein patterns and gradients

How the three Min proteins self-organize to form patterns, their response to geometric boundaries, and how these patterns can in turn induce patterns of other molecules are reviewed, focusing primarily on experimental approaches and developments.

High-throughput imaging and quantitative analysis uncovers the nature of plasmid positioning by ParABS

The results indicate that ParABS regularly positions plasmids across the nucleoid but operates just below the threshold of an oscillatory instability, minimising ATP consumption.



PomZ, a ParA‐like protein, regulates Z‐ring formation and cell division in Myxococcus xanthus

It is shown that lack of the ParA‐like protein PomZ in Myxococcus xanthus resulted in division defects with the formation of chromosome‐free minicells and filamentous cells, and the data suggest that PomZ is part of a system that recruits FtsZ to midcell, thereby, restricting Z‐ring formation to this position.

Cell cycle coordination and regulation of bacterial chromosome segregation dynamics by polarly localized proteins

It is shown in Caulobacter crescentus that the polarity factor TipN regulates the directional motion and overall translocation speed of the parS/ParB partition complex by interacting with ParA at the new pole, suggesting a mechanistic framework for adapting a self‐organizing oscillator to create motion suitable for chromosome segregation.

A spindle-like apparatus guides bacterial chromosome segregation

A bacterial chromosome segregation mechanism that features basic operating principles similar to eukaryotic mitotic machines is elucidated, including a multivalent protein complex at the centromere that stimulates the dynamic disassembly of polymers to move chromosomes into daughter compartments.

Directed and persistent movement arises from mechanochemistry of the ParA/ParB system

It is shown that the ParA/ParB system can work as a Brownian ratchet, which effectively couples the ATPase-dependent cycling of ParA–nucleoid affinity to the motion of the ParB-bound cargo.

A propagating ATPase gradient drives transport of surface-confined cellular cargo

A cell-free reconstitution of the F-plasmid partition system supports a non–filament-based mode of transport that may be as widely found in nature as filament-based mechanisms, and establishes a distinct class of motor system used for the transport and positioning of large cellular cargo.

FtsZ ring structure associated with division in Escherichia coli

The results suggest that FtsZ self-assembles into a ring structure at the future division site and may function as a cytoskeletal element and the formation of this ring may be the point at which division is regulated.

Cell-free study of F plasmid partition provides evidence for cargo transport by a diffusion-ratchet mechanism

The findings favor a diffusion-ratchet model for plasmid motion whereby partition complexes create an ATPase concentration gradient and then climb up this gradient toward higher concentrations of the ATPase.

Tracking of Chromosome and Replisome Dynamics in Myxococcus xanthus Reveals a Novel Chromosome Arrangement

The spatial arrangement and temporal dynamics of the 9.1 Mb circular chromosome in the rod-shaped cells of Myxococcus xanthus are analyzed and it is concluded that in newborn cells ori and ter regions are localized in the subpolar regions of the old and new cell pole, respectively and each separated from the nearest pole by approximately 1 µm.

Pattern formation in Escherichia coli: A model for the pole-to-pole oscillations of Min proteins and the localization of the division site

Using computer simulations, it is shown that Min protein dynamics can be described accurately by using the following assumptions: the MinD ATPase self-assembles on the membrane and recruits both MinC, an inhibitor of Z ring formation, and MinE, a protein required for MinC/MinD oscillation.