The Pleurotus ostreatus laccase multi-gene family: isolation and heterologous expression of new family members

@article{Pezzella2008ThePO,
  title={The Pleurotus ostreatus laccase multi-gene family: isolation and heterologous expression of new family members},
  author={Cinzia Pezzella and Flavia Autore and Paola Giardina and Alessandra Piscitelli and Giovanni Sannia and Vincenza Faraco},
  journal={Current Genetics},
  year={2008},
  volume={55},
  pages={45-57}
}
This work was aimed at identifying and at characterizing new Pleurotus ostreatus laccases, in order to individuate the most suitable biocatalysts for specific applications. The existence of a laccase gene clustering was demonstrated in this basidiomycete fungus, and three new laccase genes were cloned, taking advantage of their closely related spatial organization on the fungus genome. cDNAs coding for two of the new laccases were isolated and expressed in the yeasts Saccharomyces cerevisiae… CONTINUE READING
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