The Pks13/FadD32 crosstalk for the biosynthesis of mycolic acids in Mycobacterium tuberculosis.

@article{Gavalda2009ThePC,
  title={The Pks13/FadD32 crosstalk for the biosynthesis of mycolic acids in Mycobacterium tuberculosis.},
  author={Sabine Gavalda and Mathieu Serge L{\'e}ger and Beno{\^i}t van der Rest and Alexandre Stella and Fabienne Bardou and Henri Montrozier and Christian Chalut and Odile Burlet-Schiltz and Hedia Marrakchi and Mamadou Daff{\'e} and Anna{\"i}k Qu{\'e}mard},
  journal={The Journal of biological chemistry},
  year={2009},
  volume={284 29},
  pages={
          19255-64
        }
}
The last steps of the biosynthesis of mycolic acids, essential and specific lipids of Mycobacterium tuberculosis and related bacteria, are catalyzed by proteins encoded by the fadD32-pks13-accD4 cluster. Here, we produced and purified an active form of the Pks13 polyketide synthase, with a phosphopantetheinyl (P-pant) arm at both positions Ser-55 and Ser-1266 of its two acyl carrier protein (ACP) domains. Combination of liquid chromatography-tandem mass spectrometry of protein tryptic digests… CONTINUE READING
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