The PiggyBac transposon enhances the frequency of CHO stable cell line generation and yields recombinant lines with superior productivity and stability.

@article{Matasci2011ThePT,
  title={The PiggyBac transposon enhances the frequency of CHO stable cell line generation and yields recombinant lines with superior productivity and stability.},
  author={Mattia Matasci and L. Baldi and David L. Hacker and F. M. Wurm},
  journal={Biotechnology and bioengineering},
  year={2011},
  volume={108 9},
  pages={2141-50}
}
Generating stable, high-producing mammalian cell lines is a major bottleneck in the manufacture of recombinant therapeutic proteins. Conventional gene transfer methods for cell line generation rely on random plasmid integration, resulting in unpredictable and highly variable levels of transgene expression. As a consequence, a large number of stably… CONTINUE READING