The Pichia pastoris dihydroxyacetone kinase is a PTS1-containing, but cytosolic, protein that is essential for growth on methanol.

@article{Lers1998ThePP,
  title={The Pichia pastoris dihydroxyacetone kinase is a PTS1-containing, but cytosolic, protein that is essential for growth on methanol.},
  author={Georg H. L{\"u}ers and Reuben Advani and Thibaut J. Wenzel and Suchitra Subramani},
  journal={Yeast},
  year={1998},
  volume={14 8},
  pages={
          759-71
        }
}
Dihydroxyacetone kinase (DAK) is essential for methanol assimilation in methylotrophic yeasts. We have cloned the DAK gene from Pichia pastoris by functional complementation of a mutant that was unable to grow on methanol. An open reading frame of 1824 bp was identified that encodes a 65.3 kDa protein with high homology to DAK from Saccharomyces cerevisiae. Although DAK from P. pastoris contained a C-terminal tripeptide, TKL, which we showed can act as a peroxisomal targeting signal when fused… CONTINUE READING

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References

Publications referenced by this paper.
SHOWING 1-10 OF 36 REFERENCES

Analysis of the carboxy-terminal peroxisomal targeting signal 1 in a homologous context in Saccharomyces cerevisiae

Y. Elgersma, A. Vos, M van den Berg
  • J. Biol
  • 1996
VIEW 3 EXCERPTS
HIGHLY INFLUENTIAL

Labeling of peroxisomes with green fluorescent protein in living P. pastoris cells.

  • The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
  • 1996
VIEW 3 EXCERPTS