The Pichia pastoris dihydroxyacetone kinase is a PTS1‐containing, but cytosolic, protein that is essential for growth on methanol

@article{Lers1998ThePP,
  title={The Pichia pastoris dihydroxyacetone kinase is a PTS1‐containing, but cytosolic, protein that is essential for growth on methanol},
  author={G. L{\"u}ers and R. Advani and T. Wenzel and S. Subramani},
  journal={Yeast},
  year={1998},
  volume={14}
}
  • G. Lüers, R. Advani, +1 author S. Subramani
  • Published 1998
  • Medicine, Biology
  • Yeast
  • Dihydroxyacetone kinase (DAK) is essential for methanol assimilation in methylotrophic yeasts. We have cloned the DAK gene from Pichia pastoris by functional complementation of a mutant that was unable to grow on methanol. An open reading frame of 1824 bp was identified that encodes a 65·3 kDa protein with high homology to DAK from Saccharomyces cerevisiae. Although DAK from P. pastoris contained a C‐terminal tripeptide, TKL, which we showed can act as a peroxisomal targeting signal when fused… CONTINUE READING
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