The Parasitic Plant Genome Project: New Tools for Understanding the Biology of Orobanche and Striga

@inproceedings{Westwood2012ThePP,
  title={The Parasitic Plant Genome Project: New Tools for Understanding the Biology of Orobanche and Striga},
  author={James H. Westwood and Claude W. dePamphilis and Malay Das and M{\'o}nica Fern{\'a}ndez-Aparicio and Loren A. Honaas and Michael P. Timko and Eric K Wafula and Norman J. Wickett and John I Yoder},
  booktitle={Weed Science},
  year={2012}
}
Abstract The Parasitic Plant Genome Project has sequenced transcripts from three parasitic species and a nonparasitic relative in the Orobanchaceae with the goal of understanding genetic changes associated with parasitism. The species studied span the trophic spectrum from free-living nonparasite to obligate holoparasite. Parasitic species used were Triphysaria versicolor, a photosynthetically competent species that opportunistically parasitizes roots of neighboring plants; Striga hermonthica… 
View on Cambridge Press
cwd.huck.psu.edu
107 Citations
Highly Influential Citations
8
Background Citations
50
Methods Citations
13
Results Citations
1

107 Citations

Citation Type

Citation Type

Horizontal gene transfer studies in parasitic plants of the orobanchaceae
TLDR
This report summarizes the HGT findings in the PPGP (The Parasitic Plant Genome Project) by utilizing a phylogenomic approach in identifying HGT events and identified two high confidence HGT transgenes in Striga hermonthica located adjacent to each other.
Functional genomics of a generalist parasitic plant: Laser microdissection of host-parasite interface reveals host-specific patterns of parasite gene expression
TLDR
De novo assembled transcriptomes generated from laser micro-dissected tissues at the host-parasite interface are compared to uncover details of the largely uncharacterized interaction between parasitic plants and their hosts to suggest T. versicolor's generalist strategy involves a reliance on overlapping but distinct gene sets, depending upon the host plant it is parasitizing.
Parasitic Plants Striga and Phelipanche Dependent upon Exogenous Strigolactones for Germination Have Retained Genes for Strigolactone Biosynthesis
TLDR
Results indicate that Phelipanche aegyptiaca and S. hermonthica have retained functional genes involved in strigolactone biosynthesis, suggesting that the parasites use both endogenous and exogenous striglactones and have mechanisms to differentiate the two.
Evolution of a horizontally acquired legume gene, albumin 1, in the parasitic plant Phelipanche aegyptiaca and related species
TLDR
The HGT-derived albumins in Phelipanche represent a novel example of how plants can acquire genes from other plants via HGT that then go on to duplicate, evolve, and retain the specialized features required to perform a unique host-derived function.
Trans-specific gene silencing of acetyl-CoA carboxylase in a root-parasitic plant.
TLDR
This work shows that ACCase is an effective target for inactivation in parasitic plants by trans-specific gene silencing in Triphysaria spp.
Transcriptomics of host-specific interactions in natural populations of the parasitic plant purple witchweed (Striga hermonthica)
TLDR
This study shows how signals of host-specific processes can be detected aboveground, expanding the focus of host–parasite interactions beyond the haustorial connection.
Seed germination in parasitic plants: what insights can we expect from strigolactone research?
TLDR
This review attempts to illustrate to what extent conclusions from research into strigolactones could be applied to better understand the biology of parasitic plants.
Root parasitic plant Orobanche aegyptiaca and shoot parasitic plant Cuscuta australis obtained Brassicaceae-specific strictosidine synthase-like genes by horizontal gene transfer
TLDR
This study strongly supports that parasitic plants can gain novel nuclear genes from distantly related host species by HGT and the foreign genes may execute certain functions in the new hosts.
Genomic signatures of host-specific selection in a parasitic plant
TLDR
This study demonstrates the utility of WGS for plant species with large, complex genomes and no available reference, suggesting recurring host-specific selection each generation alternating with homogenizing gene flow.
WARPP - Web Application for the Research of Parasitic Plants.
TLDR
The Web Application for the Research of Parasitic Plants (WARPP) is introduced as an online resource dedicated to advancing research and development of parasitic plant biology and is a framework to facilitate international efforts by providing a central hub of curated evolutionary, ecological, and genetic data.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 108 REFERENCES
Transcriptomes of the Parasitic Plant Family Orobanchaceae Reveal Surprising Conservation of Chlorophyll Synthesis
The Use of Arabidopsis to Study Interactions between Parasitic Angiosperms and Their Plant Hosts
TLDR
Arabidopsis is being used as a model host plant to identify genetic loci associated with stimulating parasite germination, haustorium development, and parasite support and how host plants respond to parasite attack.
Characterization of the Orobanche–Arabidopsis system for studying parasite–host interactions
TLDR
Experiments comparing wild-type A. thaliana plants to mutant lines deficient in flavonoid biosynthesis revealed no differences in the ability to stimulate germination or to allow tubercle formation, indicating that host flavonoids production is not essential for Orobanche parasitization.
Trans-specific gene silencing between host and parasitic plants.
TLDR
It is demonstrated here that interfering hairpin constructs transformed into host plants can silence expression of the targeted genes in the parasite, providing a novel strategy for controlling parasitic weeds.
Gene silencing of mannose 6-phosphate reductase in the parasitic weed Orobanche aegyptiaca through the production of homologous dsRNA sequences in the host plant.
TLDR
The detection of mir390, which is involved with cytoplasmic dsRNA processing, is the first indication of the existence of gene-silencing mechanisms in Orobanche spp.
Host‐driven divergence in the parasitic plant Orobanche minor Sm. (Orobanchaceae)
TLDR
Results show that O. minor sensu lato may comprise distinct, genetically divergent races isolated by the different ecologies of their hosts, and suggest that host specificity may be an important driver of allopatric speciation in parasitic plants.
Gene expression profiling of Medicago truncatula roots in response to the parasitic plant Orobanche crenata
TLDR
Gene expression patterns suggest that resistance mechanisms activated in both genotypes are temporally and spatially different and resemble those associated with plant resistance to microbial pathogens.
PlantTribes: a gene and gene family resource for comparative genomics in plants
TLDR
The PlantTribes database was used as a scaffold for sorting ∼4 million additional cDNA sequences from over 200 plant species, and SuperTribes, constructed through a second iteration of MCL clustering, connect distant, but potentially related gene clusters.
Candidate effector gene identification in the ascomycete fungal phytopathogen Venturia inaequalis by expressed sequence tag analysis.
TLDR
Sixteen new candidate effector genes were identified from V. inaequalis, which conformed to most of the protein structural characteristics expected of fungal effectors and showed significant levels of transcriptional up-regulation during in planta growth.
Host-specific races in the holoparasitic angiosperm Orobanche minor: implications for speciation in parasitic plants.
TLDR
The host specificity of natural populations of O. minor is quantified for the first time, and evidence that this species may comprise distinct physiological races is provided, speculated to isolate races of Orobanche on different hosts, accelerating divergence and ultimately speciation in this genus.
...
1
2
3
4
5
...