The POZ/BTB protein NAC1 interacts with two different histone deacetylases in neuronal‐like cultures

@article{Korutla2005ThePP,
  title={The POZ/BTB protein NAC1 interacts with two different histone deacetylases in neuronal‐like cultures},
  author={L. Korutla and P. J. Wang and S. Mackler},
  journal={Journal of Neurochemistry},
  year={2005},
  volume={94}
}
NAC1 is a cocaine‐regulated POZ/BTB (Pox virus and Zinc finger/Bric‐a‐brac Tramtrack Broad complex) protein. NAC1 is increased by cocaine selectively in the nucleus accumbens, a CNS region important for drug addiction. NAC1's role in the cell, however, is not known. Each of the two NAC1 isoforms, sNAC1 (short NAC1) and lNAC1 (long NAC1), may serve as corepressors for other POZ/BTB proteins. This study investigated whether sNAC1 and lNAC1 demonstrated protein–protein interactions with other… Expand
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  • M. A. Stead, S. Carr, S. Wright
  • Biology, Medicine
  • Acta crystallographica. Section F, Structural biology and crystallization communications
  • 2009
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References

SHOWING 1-10 OF 30 REFERENCES
Corepressor SMRT binds the BTB/POZ repressing domain of the LAZ3/BCL6 oncoprotein.
TLDR
It is suggested that LAZ3/BCL6 and nuclear hormone receptors repress transcription through shared mechanisms involving SMRT recruitment and histone deacetylation. Expand
Recruitment of SMRT/N-CoR-mSin3A-HDAC-repressing complexes is not a general mechanism for BTB/POZ transcriptional repressors: the case of HIC-1 and gammaFBP-B.
TLDR
The studies show that the recruitment onto target promoters of an HDAC complex is not a general property of transcriptional repressors containing a conserved BTB/POZ domain. Expand
NAC-1 Is a Brain POZ/BTB Protein That Can Prevent Cocaine-Induced Sensitization in the Rat
TLDR
Adenoviral-mediated overexpression of NAC-1 protein in the rat nucleus accumbens prevented the development but not the expression of behavioral sensitization produced by repeated administration of cocaine, suggesting that Nac-1 may modify the long-term behaviors of psychostimulant abuse by regulating gene transcription in the mammalian brain. Expand
Histone deacetylase associated with mSin3A mediates repression by the acute promyelocytic leukemia-associated PLZF protein
TLDR
A role for deregulated histone deacetylation in the development of both lymphoid and myeloid neoplasia in human and suggests that targeted histoneDeacetylase inhibitors may be useful for treatment of certain types of malignancies is supported. Expand
The BCL-6 POZ domain and other POZ domains interact with the co-repressors N-CoR and SMRT
TLDR
The demonstration that N-CoR and SMRT interact with the POZ domain containing proteins indicates that these co-repressors are likely involved in the mediation of repression by multiple classes of repressors and may explain, in part, how POz domain containing repressor mediate transcriptional repression. Expand
A new functional domain of Bcl6 family that recruits histone deacetylases.
TLDR
Results indicate that the 17aa region in the middle portion of Bcl6 is a functional domain of transrepressor activity and is responsible for inducibility of apoptosis in NIH3T3 cells. Expand
The LAZ3(BCL-6) oncoprotein recruits a SMRT/mSIN3A/histone deacetylase containing complex to mediate transcriptional repression.
TLDR
It is concluded that LAZ3 recruits a repressing complex containing SMRT, mSIN3A and a HDAC, and that its full repressing potential on transcription requires HDACs activity. Expand
Differences in expression, actions and cocaine regulation of two isoforms for the brain transcriptional regulator NAC1
TLDR
It is concluded that the two isoforms of NAC1 may differentially affect neuronal functions, including the regulation of cocaine-induced locomotion, in the rat brain and peripheral tissues. Expand
Ski is a component of the histone deacetylase complex required for transcriptional repression by Mad and thyroid hormone receptor.
TLDR
The involvement of c-Ski in theHDAC complex indicates that the function of the HDAC complex is important for oncogenesis and that Ski is required for the transcriptional repression mediated by this complex. Expand
Neuronal activity‐dependent nucleocytoplasmic shuttling of HDAC4 and HDAC5
TLDR
It is found that, in cultured hippocampal neurones, the localization of HDAC4 and HDAC5 is dynamic and signal‐regulated, which provides a mechanism for input‐specific gene expression. Expand
...
1
2
3
...