The PI 3 K / Akt Pathway Regulates Oxygen Metabolism via Pyruvate Dehydrogenase ( PDH )-E 1 a Phosphorylation

Inhibition of the PI3K/Akt pathway decreases hypoxia within SQ20B human head and neck cancer xenografts. We set out to understand the molecular mechanism underlying this observation. We measured oxygen consumption using both a Clark electrode and an extracellular flux analyzer. We made these measurements after various pharmacologic and genetic manipulations… (More)