The P1 plasmid partition protein ParA. A role for ATP in site-specific DNA binding.

@article{Davey1994ThePP,
  title={The P1 plasmid partition protein ParA. A role for ATP in site-specific DNA binding.},
  author={Megan J. Davey and Barbara E. Funnell},
  journal={The Journal of biological chemistry},
  year={1994},
  volume={269 47},
  pages={29908-13}
}
ParA, a P1 protein required for partition of the prophage plasmid, regulates expression of its own gene and another partition gene, parB, from a promoter upstream of parA. The ATP-dependent ParA DNA binding activity to the par promoter is thought to mediate this regulation. An alternate purification for ParA is presented. This highly purified ParA was used to examine ParA DNA binding activity using DNase I protection assays. At high concentration, ParA bound to the par promoter in the absence… CONTINUE READING

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