The Overproduction of Leucyl-tRNA Synthetase in E. coli and Its Purification.

Abstract

The 3.2 kb gene (leuS) encoding for leucyl-tRNA synthetase (LeuRS) has been cloned from E.coli K-12, and overexpressed 35 times more than that in the host strain TG1. In order to further increase the production of LeuRS, two types of leuS with different length of the 3' flanking region: leuS1 has an additional 130 bp over that of leuS2 were ligated into pKK… (More)

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@article{Li1997TheOO, title={The Overproduction of Leucyl-tRNA Synthetase in E. coli and Its Purification.}, author={Tong Li and En-Duo Wang and Ying-Lai Wang}, journal={Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica}, year={1997}, volume={29 6}, pages={591-596} }