The Organotypic Longitudinal Spinal Cord Slice Culture for Stem Cell Study

  title={The Organotypic Longitudinal Spinal Cord Slice Culture for Stem Cell Study},
  author={Joanna Sypecka and Sylwia Koniusz and Maria Kawalec and Anna Sarnowska},
  booktitle={Stem cells international},
The objective of this paper is to describe in detail the method of organotypic longitudinal spinal cord slice culture and the scientific basis for its potential utility. The technique is based on the interface method, which was described previously and thereafter was modified in our laboratory. The most important advantage of the presented model is the preservation of the intrinsic spinal cord fiber tract and the ventrodorsal polarity of the spinal cord. All the processes occurring during… CONTINUE READING
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