The Occurrence of 7-Aminobutyric Acid (GABA)-Containing Cells in Cultures of Retinas from the Human Fetus

Abstract

1. γ-Aminobutyric acid (GABA) immunoreactivity is demonstrated by the indirect immunofluorescence technique in a small population of retinal neurons cultured from human fetuses. 2. Positive staining was restricted to a few cells and could be observed as soon as the cells became attached to the substrate (within 5 hr). It is therefore concluded that the GABA-positive cells are determined prenatally. 3. The GABA-positive cells grow processes during development in culture and remain constant in numbers. These cells have a different morphology from either GFAP-positive cells or serotinin-accumulating cells. 4. It is suggested that the GABA-positive cells in culture are probably amacrine neurones. 5. Cultures of human retinal dissociates may therefore provide an alternative means of studying specific cell types should a constant supply of living human retinas be difficult to obtain. γ-Aminobutyric acid (GABA) immunoreactivity is demonstrated by the indirect immunofluorescence technique in a small population of retinal neurons cultured from human fetuses. Positive staining was restricted to a few cells and could be observed as soon as the cells became attached to the substrate (within 5 hr). It is therefore concluded that the GABA-positive cells are determined prenatally. The GABA-positive cells grow processes during development in culture and remain constant in numbers. These cells have a different morphology from either GFAP-positive cells or serotinin-accumulating cells. It is suggested that the GABA-positive cells in culture are probably amacrine neurones. Cultures of human retinal dissociates may therefore provide an alternative means of studying specific cell types should a constant supply of living human retinas be difficult to obtain.

DOI: 10.1007/BF00742978

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Cite this paper

@article{Osborne1986TheOO, title={The Occurrence of 7-Aminobutyric Acid (GABA)-Containing Cells in Cultures of Retinas from the Human Fetus}, author={Neville N. Osborne and Deirdre Beaton}, journal={Cellular and Molecular Neurobiology}, year={1986}, volume={6}, pages={87-93} }