The Nuclear Pore-Associated TREX-2 Complex Employs Mediator to Regulate Gene Expression

@article{Schneider2015TheNP,
  title={The Nuclear Pore-Associated TREX-2 Complex Employs Mediator to Regulate Gene Expression},
  author={M. Schneider and D. Hellerschmied and Tobias Schubert and Stefan Amlacher and Vinesh Vinayachandran and Rohit Reja and B. Pugh and T. Clausen and A. K{\"o}hler},
  journal={Cell},
  year={2015},
  volume={162},
  pages={1016 - 1028}
}
Summary Nuclear pore complexes (NPCs) influence gene expression besides their established function in nuclear transport. The TREX-2 complex localizes to the NPC basket and affects gene-NPC interactions, transcription, and mRNA export. How TREX-2 regulates the gene expression machinery is unknown. Here, we show that TREX-2 interacts with the Mediator complex, an essential regulator of RNA Polymerase (Pol) II. Structural and biochemical studies identify a conserved region on TREX-2, which… Expand
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  • Chemistry, Medicine
  • Sub-cellular biochemistry
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TLDR
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An Auxin-Induced Degron (AID) system is applied to distinguish roles of basket nucleoporins NUP153, NUP50 and TPR to demonstrate a unique and pivotal role of TPR in gene expression through TREX-2- and/or NXF1-dependent mRNA turnover. Expand
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Nuclear export of messenger ribonucleic acid (mRNA) is a complex and essential process for a correct gene expression in all eukaryotic cells. The export of mRNA through the nuclear pore complexExpand
Transcription regulation by the Mediator complex
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  • Biology, Medicine
  • Nature Reviews Molecular Cell Biology
  • 2018
TLDR
Novel roles of Mediator in the control of gene expression have been revealed by showing its connection to the nuclear pore and linking Mediator to the regulation of gene positioning in the nuclear space. Expand
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