The NosX and NirX proteins of Paracoccus denitrificans are functional homologues: their role in maturation of nitrous oxide reductase.

@article{Saunders2000TheNA,
  title={The NosX and NirX proteins of Paracoccus denitrificans are functional homologues: their role in maturation of nitrous oxide reductase.},
  author={Neil F. W. Saunders and Jorrit J Hornberg and Willem N. M. Reijnders and Hans V. Westerhoff and Simon de Vries and Rob J. M. van Spanning},
  journal={Journal of bacteriology},
  year={2000},
  volume={182 18},
  pages={5211-7}
}
The nos (nitrous oxide reductase) operon of Paracoccus denitrificans contains a nosX gene homologous to those found in the nos operons of other denitrifiers. NosX is also homologous to NirX, which is so far unique to P. denitrificans. Single mutations of these genes did not result in any apparent phenotype, but a double nosX nirX mutant was unable to reduce nitrous oxide. Promoter-lacZ assays and immunoblotting against nitrous oxide reductase showed that the defect was not due to failure of… CONTINUE READING

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