The Nitrogen‐Fixing System of Corynebacterium autotrophicum

@article{Berndt1978TheNS,
  title={The Nitrogen‐Fixing System of Corynebacterium autotrophicum},
  author={H. Berndt and David J. Lowe and M. Geoffrey Yates},
  journal={FEBS Journal},
  year={1978},
  volume={86},
  pages={133-142}
}
Both nitrogenase components were isolated and purified from sucrose-grown, nitrogen-fixing cells of Corynebacterium autotrophicum by DEAE-cellulose and Sephadex gel chromatography. The MoFe protein (Ca1) and the Fe protein (Ca2) appeared as single bands in sodium dodecylsulfate gel electrophoresis (Serva). Ca1 had an average molecular weight of 232000 with two non-identical subunits (Mr 61200 and 57500) on Koch-Light sodium dodecylsulfate gels; the Ca2 protein had an average molecular weight of… Expand
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