The Nature of Negative Cooperativity in Alkaline Phosphatase

@inproceedings{R2001TheNO,
  title={The Nature of Negative Cooperativity in Alkaline Phosphatase},
  author={R. and P................... and Y.},
  year={2001}
}
The nature of strong negative cooperativity displayed by alkaline phosphatase from Escherichia coli was investigated by alternative substrate and product inhibition studies, and by catalytic rate constant (kcat) measurements. To see whether the idled subunit in this dimeric enzyme plays a mechanistic role in the overall catalysis, the flip-flop model… CONTINUE READING