The N-terminal domain of mammalian Lysyl-tRNA synthetase is a functional tRNA-binding domain.

@article{Francin2002TheND,
  title={The N-terminal domain of mammalian Lysyl-tRNA synthetase is a functional tRNA-binding domain.},
  author={Mathilde Francin and Monika Kamińska and Pierre Kerjan and Marc Mirande},
  journal={The Journal of biological chemistry},
  year={2002},
  volume={277 3},
  pages={1762-9}
}
Lysyl-tRNA synthetase from higher eukaryotes possesses a lysine-rich N-terminal polypeptide extension appended to a classical prokaryotic-like LysRS domain. Band shift analysis showed that this extra domain provides LysRS with nonspecific tRNA binding properties. A N-terminally truncated derivative of LysRS, LysRS-DeltaN, displayed a 100-fold lower apparent affinity for tRNA(3)Lys and a 3-fold increase in K(m) for tRNA(3)Lys in the aminoacylation reaction, as compared with the native enzyme… CONTINUE READING
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