The Multiwavelength Anomalous Solvent Contrast (MASC) Method in Macromolecular Crystallography.

@article{Fourme1995TheMA,
  title={The Multiwavelength Anomalous Solvent Contrast (MASC) Method in Macromolecular Crystallography.},
  author={Roger Fourme and William E Shepard and Richard A. Kahn and G L'hermite and I M Li de la Sierra},
  journal={Journal of synchrotron radiation},
  year={1995},
  volume={2 Pt 1},
  pages={
          36-48
        }
}
The wavelength dependence of anomalous scattering of X-rays, due to atoms randomly dispersed in the solvent phase of a macromolecular crystal, is a way of producing solvent-density contrast variation with perfect isomorphism. The largest contrast variations are obtained by tuning the X-ray wavelength near an absorption edge of the anomalous-scattering species. In this method, which we call MASC, the anomalous partial structure is an extended uniform electron density, in contrast to the few… 
Anomalous signal of solvent bromides used for phasing of lysozyme.
TLDR
The anomalous signal of bromide ions, present in the crystal structure of tetragonal hen egg-white lysozyme through the substitution of NaCl by NaBr in the crystallization medium, was used for phasing of X-ray data, leading to easily interpretable electron density.
The Solvent Contrast Variation Method
The distinctly non-uniform distribution of atomic positions created by solvent channels in the asymmetric units of protein crystals is a defining distinction between small molecules and
Determination of protein and solvent volumes in protein crystals from contrast variation data.
  • J. Badger
  • Chemistry, Medicine
    Basic life sciences
  • 1996
TLDR
Trial applications to two problems in crystal structure determination illustrate howNeutron diffraction methods are ideally suited to these contrast variation experiments and an ab initio phasing method for the contrast variation data was found to recover the correct molecular envelope.
Small-angle scattering and its interplay with crystallography, contrast variation in SAXS and SANS.
  • H. Stuhrmann
  • Chemistry, Medicine
    Acta crystallographica. Section A, Foundations of crystallography
  • 2008
TLDR
Methods of contrast variation are tools that are essential in macromolecular structure research and recent attempts to extend this method to native resonant labels like sulfur and phosphorus are promising.
Iterative projection algorithms and applications in x-ray crystallography
  • V. Lo
  • Computer Science
  • 2011
X-ray crystallography is a technique for determining the structure (positions of atoms in space) of molecules. It is a well developed technique, and is applied routinely to both small inorganic and
Invited original talk Anomalous diffraction with soft X-ray synchrotron radiation: DANES from pentakismethylammonium undecachlorodibismuthate at the K absorption edge of chlorine
Soft X-ray diffraction from synchrotron radiation gives access to the use of MAD (multi-wavelength anomalous diffraction) and DAFS / DANES (diffraction anomalous fine structure / diffraction near
Detergent binding in trigonal crystals of OmpF porin from Escherichia coli.
TLDR
The structure of the detergent, ocytyl hydroxyethylsufoxide (C8(HE)SO), bound to the OmpF porin from E coli (in the trigonal crystal form), determined by neutron crystallography strongly suggests that the same areas define, in vivo, the location at which protein interacts with lipid.
Protein–phospholipid interplay revealed with crystals of a calcium pump
The lipid bilayer has so far eluded visualization by conventional crystallographic methods, severely limiting our understanding of phospholipid– and protein–phospholipid interactions. Here we
The prominent role of resonant elastic scattering for solving the X-ray structure of macromolecules
This article is a short overview of basic principles, methods and instrumentation of phasing methods based on anomalous scattering for X-ray macromolecular crystallography (MX).
SOLVING A HEAVY-ATOM SUBSTRUCTURE BY DIRECT METHODS
TLDR
This work has shown that statistical coupling between structure factors within a single dataset can be used to refine phases from random starting phase sets or from a partial structure obtained, for instance by Patterson methods.
...
1
2
...

References

SHOWING 1-10 OF 16 REFERENCES
Biophysics and Synchrotron Radiation
This book the second volume in the "Springer Series in Biophysics" col lects together contributions to the conference on "Biophysics and Syn chrotron Radiation" held in July 86 at Frascati. This
Biophysical Chemistry: Part II: Techniques for the Study of Biological Structure and Function
Characterization of Cereals and Flours is a state-of-theart reference that details the latest advances to characterize the effects of manufacturing processes and storage conditions on the thermal,
"J."
however (for it was the literal soul of the life of the Redeemer, John xv. io), is the peculiar token of fellowship with the Redeemer. That love to God (what is meant here is not God’s love to men)
Acta Cryst
  • A46, 57--68. Crumley, K. V. (1989). MSc thesis, Univ. of North Carolina,
  • 1990
PhD thesis, Univ
  • of Hamburg, Germany.
  • 1993
European Patent Application
  • 1992
PhD thesis, Univ
  • of Hamburg, Germany.
  • 1988
Th~se de doctorat et sciences naturelles
  • 1988
In Anomalous Scattering Factors for Synchro
  • 1984
In Anomalous Scattering Factors for Synchrotron Radiation Users Calculated using the Cromer and Liberman Method
  • 1984
...
1
2
...