The Minimum Vitamin Requirements of the L and Hela Cells in Tissue Culture, the Production of Specific Vitamin Deficiencies, and Their Cure

@article{Eagle1955TheMV,
  title={The Minimum Vitamin Requirements of the L and Hela Cells in Tissue Culture, the Production of Specific Vitamin Deficiencies, and Their Cure},
  author={Harry Eagle},
  journal={The Journal of Experimental Medicine},
  year={1955},
  volume={102},
  pages={595 - 600}
}
  • Harry Eagle
  • Published 1955 in The Journal of experimental medicine
SEVEN VITAMINS HAVE TO DATE PROVED ESSENTIAL FOR THE SURVIVAL AND MULTIPLICATION OF A MOUSE FIBROBLAST (STRAIN L) AND A HUMAN CARCINOMA CELL (STRAIN HELA) IN TISSUE CULTURE: choline, folic acid, nicotinamide, pantothenic acid, pyridoxal, riboflavin, and thiamin. It was necessary to cultivate the cells for 5 to 15 days in a medium lacking the specific vitamin before the deficiency became apparent in the cessation of multiplication and the development of specific cytopathogenic effects. In their… CONTINUE READING