The Mechanics of Injury to Isolated Protoplasts following Osmotic Contraction and Expansion.

@article{Dowgert1987TheMO,
  title={The Mechanics of Injury to Isolated Protoplasts following Osmotic Contraction and Expansion.},
  author={M F Dowgert and Joe Wolfe and Peter L. Steponkus},
  journal={Plant physiology},
  year={1987},
  volume={83 4},
  pages={
          1001-7
        }
}
Micro-osmotic manipulation was used to determine the influence of osmotic contraction on the expansion potential of individual protoplasts isolated from rye (Secale cereale L. cv Puma) leaves. For protoplasts isolated from leaves of nonacclimated plants (NA protoplasts), osmotic contraction in sufficiently hypertonic solutions (>1.53 osmolal) predisposed the protoplasts to lysis during osmotic expansion when they were returned to isotonic conditions (0.53 osmolal). In contrast, for protoplasts… CONTINUE READING

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