The Measurement of Swimming Velocity of Vibrio cholerae and Pseudomonas aeruginosa Using the Video Tracking Method

@article{Shigematsu1995TheMO,
  title={The Measurement of Swimming Velocity of Vibrio cholerae and Pseudomonas aeruginosa Using the Video Tracking Method},
  author={M Shigematsu and Yuko Meno and Hitoko Misumi and Kazunobu Amako},
  journal={Microbiology and Immunology},
  year={1995},
  volume={39}
}
The swimming velocities of two monotrichous flagellated bacteria were measured by a computer‐assisted video tracking method. Tracing the moving path of the individual bacterium revealed that the bacterial cell did not swim continuously in a straight direction, but frequently changed swimming direction and velocity. The average swimming velocities calculated from the 3‐sec path were 75.4 ±9.4 μm/sec in four strains of Vibrio cholerae and 513 ±8.4 μm/sec in five strains of Pseudomonas aeruginosa… Expand

Topics from this paper

Swimming Behavior of Pseudomonas aeruginosa Studied by Holographic 3D Tracking
TLDR
It is shown that Pseudomonas aeruginosa performs helical movements which were so far only described for larger microorganisms. Expand
Spirochaete-like swimming mode of Campylobacter jejuni in a viscous environment.
TLDR
The spiral shape of this bacterium like that of spirochaetes may strongly influence its swimming ability in media of high viscosity such as the mucous layer of the intestinal tract. Expand
Asymmetric swimming pattern of Vibrio alginolyticus cells with single polar flagella.
TLDR
The swimming pattern of bacteria with single polar flagella with monotrichously flagellated Vibrio alginolyticus cells is observed; the cells were found to curve sharply during backward swimming, while they progressed linearly during forward swimming. Expand
Physics of microorganism behaviour: motility, synchronisation, run-and-tumble, phototaxis.
Microorganisms have evolved in a low Reynolds number environment and have adapted their behaviour to its viscosity. Here, we consider some features of behaviour observed in microorganisms and useExpand
High density waves of the bacterium Pseudomonas aeruginosa in propagating swarms result in efficient colonization of surfaces.
TLDR
A new strategy of efficient colonization and community development where bacteria substantially alter their physical environment is described where P. aeruginosa responds to environmental cues on a very short timescale by actively exploiting local physical phenomena to develop communities and efficiently colonize new surfaces. Expand
CO$_2$-driven diffusiophoresis for removal of bacteria
We investigate CO$_2$-driven diffusiophoresis of colloidal particles and bacterial cells in a Hele-Shaw geometry. Combining experiments and a model, we understand the characteristic length and timeExpand
Spirochete Flagella and Motility
TLDR
Diverse morphology and motility observed among the spirochetes are described and the current knowledge on their mechanisms and relations to pathogenicity is summarized, mainly from the standpoint of experimental biophysics. Expand
Numerical study on the cell motility interacting with the chemical flow in microchannels
Beneficial to the steady control of flow properties and concentration gradient profiles, quantification of cell chemotaxis based on microfluidic devices could achieve on the scale of a single cell.Expand
CO2-Driven diffusiophoresis for maintaining a bacteria-free surface.
TLDR
Long-time experiments suggest potential applications for bacterial diffusiophoresis to cleaning systems or anti-biofouling surfaces, by reducing the population of the cells near CO2 sources, as well as identifying the characteristic length and time scales of CO2-driven diffusing in relation to system dimensions and CO2 diffusivity. Expand
A model for Vibrio cholerae colonization of the human intestine.
TLDR
A mathematical model based on a convection-diffusion-reaction-swimming equation capturing bacterial dynamics coupled with Stokes equations governing fluid velocity is developed that indicates that both host and bacterial factors contribute to bacterial density in the gut. Expand
...
1
2
3
...

References

SHOWING 1-10 OF 11 REFERENCES
Motility of Campylobacter jejuni in a viscous environment: comparison with conventional rod-shaped bacteria.
TLDR
The findings of the study suggest that C. jejuni has a motility suited to movement in a viscous environment, and that this ability might provide the organism with an ecological advantage when in intestinal mucus. Expand
Effect of Viscosity on Bacterial Motility
TLDR
Theoretically derived thermodynamic equations suggest that the conformation of the flagellar helix affects efficiency of propulsion, and such a relationship between helix waveform and velocity was experimentally observed with Thiospirillum jenese. Expand
Motility of flagellated bacteria in viscous environments
The lowest viscosity that immobilized flagellated bacteria such as Psedomonas aeruginosa, Spirillum serpens, and Escherichia coli was 60 centipoise (cp). Much higher viscosities (1,000 cp and higher)Expand
The Measurement of Bacterial Motility
TLDR
Bacterial motility was measured by a simple method which consists of counting the number of organisms which pass across a small aperture in a given time according to the bacterial concentration in the suspension and also to the average speed of the bacteria. Expand
Isolation of nonchemotactic mutants of Campylobacter jejuni and their colonization of the mouse intestinal tract
TLDR
It is concluded that chemotactic movement is important for colonization of the intestinal tract of suckling mice by C. jejuni. Expand
Roles of motility and flagellar structure in pathogenicity of Vibrio cholerae: analysis of motility mutants in three animal models
TLDR
Analysis of the wild-type and mutant strains in these three animal models clearly demonstrated a role for motility in V. cholerae pathogenicity, while analysis of only the nonmotile mutants derived from the classical parent suggested a roles for flagellar structures. Expand
Movement of microorganisms in viscous environments
TLDR
Data show that solutions of methylcellulose are gel-like even when quite dilute, when the bulk viscosity is as small as 2 cP, and that solutions containing highly branched polymers, for example, Ficoll, are much more homogeneous. Expand
In vivo adherence and colonization of Vibrio cholerae strains that differ in hemagglutinating activity and motility
TLDR
It is concluded that, at least in the animal model used, factors other than hemagglutinating activity and motility may also play a role in the association of V. cholerae with the small intestinal surface. Expand
Visualization of bacterial flagella by video-enhanced light microscopy
TLDR
Individual flagellar filaments of Escherichia coli, a motile Streptococcus sp. Expand
Motility as an intestinal colonization factor for Campylobacter jejuni.
TLDR
It is concluded that motility is an important factor in the colonization of the intestinal tract of suckling mice by C. jejuni. Expand
...
1
2
...