The Leishmania kinetoplast-mitochondrion contains terminal uridylyltransferase and RNA ligase activities.

@article{Bakalara1989TheLK,
  title={The Leishmania kinetoplast-mitochondrion contains terminal uridylyltransferase and RNA ligase activities.},
  author={Norbert Bakalara and Agda M. Simpson and Larry Simpson},
  journal={The Journal of biological chemistry},
  year={1989},
  volume={264 31},
  pages={18679-86}
}
Purified mitochondria of Leishmania tarentolae contain 3'-terminal uridylyltransferase and RNA ligase activities which can be solubilized by detergent lysis of the organelle. Run-off transcription of maxicircle and minicircle DNA also occurs in intact and Triton-lysed mitochondria, using [32P]GTP as the labeled precursor. Heparin inhibits the solubilized terminal uridylyltransferase activity but does not affect the labeling of endogenous RNAs in intact mitochondria with [32P]UTP. Clarification… CONTINUE READING

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Nucleic Acids Res

  • T. White, G. Rudenko, P. Borst
  • Proc. Natl
  • 1986

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