The Ld Cht1 gene encodes the secretory chitinase of the human pathogen Leishmania donovani.


Leishmania donovani promastigotes were shown to release chitinase activity during their growth in vitro. A PCR-based strategy identified a single copy ORF capable of encoding the L. donovani chitinase (Ld Cht1, 1374 bp). Ld Cht1 was shown to be actively transcribed by L. donovani promastigotes using reverse transcription and PCR amplification. The deduced… (More)


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