The Lactococcal lmrP gene encodes a proton motive force-dependent drug transporter.

@article{Bolhuis1995TheLL,
  title={The Lactococcal lmrP gene encodes a proton motive force-dependent drug transporter.},
  author={Henk Bolhuis and Gerrit J Poelarends and Hendrik W van Veen and Bert Poolman and Arnold M. J. Driessen and Wil N. Konings},
  journal={The Journal of biological chemistry},
  year={1995},
  volume={270 44},
  pages={26092-8}
}
To genetically dissect the drug extrusion systems of Lactococcus lactis, a chromosomal DNA library was made in Escherichia coli and recombinant strains were selected for resistance to high concentrations of ethidium bromide. Recombinant strains were found to be resistant not only to ethidium bromide but also to daunomycin and tetraphenylphosphonium. The drug resistance is conferred by the lmrP gene, which encodes a hydrophobic polypeptide of 408 amino acid residues with 12 putative membrane… CONTINUE READING