The Kw recombinase, an integrase from Kluyveromyces waltii.

  title={The Kw recombinase, an integrase from Kluyveromyces waltii.},
  author={Leonie Ringrose and Pierre-Olivier Angrand and Adrian Francis Stewart},
  journal={European journal of biochemistry},
  volume={248 3},
Site-specific recombinases of the integrase family share limited amino-acid-sequence similarity, but use a common reaction mechanism to recombine distinct DNA target sites. Here we report the characterisation of the Kw site-specific recombinase, encoded on the 2 mu-like plasmid pKWS1 from the yeast Kluyveromyces waltii. Using in vitro-translated Kw recombinase, we show that the protein is able to bind and to recombine its putative DNA target site. Recombination is conservative and the Kw target… CONTINUE READING