The Ku-like protein from Saccharomyces cerevisiae is required in vitro for the assembly of a stable multiprotein complex at a eukaryotic origin of replication.

@article{Shakibai1996TheKP,
  title={The Ku-like protein from Saccharomyces cerevisiae is required in vitro for the assembly of a stable multiprotein complex at a eukaryotic origin of replication.},
  author={Nader Shakibai and Vasantha H. S. Kumar and Shlomo Eisenberg},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1996},
  volume={93 21},
  pages={
          11569-74
        }
}

• Nader Shakibai, Vasantha H. S. Kumar, Shlomo Eisenberg
• Published in
  Proceedings of the National Academy of Sciences…
  1996

We have previously shown that three distinct DNA-binding activities, in crude form, are necessary for the ATP-dependent assembly of a specific and stable multiprotein complex at a yeast origin of replication. Here we show the purification of one of these DNA binding activities, referred to as origin binding factor 2 (OBF2). The purified protein is a heterodimer composed of two polypeptides with molecular mass values of 65 and 80 kDa as determined by SDS/PAGE. Purified OBF2 not only binds DNA… CONTINUE READING

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