The IspA protease's involvement in the regulation of the sporulation process of Bacillus thuringiensis is revealed by proteomic analysis.

@article{Chen2003TheIP,
  title={The IspA protease's involvement in the regulation of the sporulation process of Bacillus thuringiensis is revealed by proteomic analysis.},
  author={F. D. Chen and Li-Fen Shen and Ming-Chu Tsai and K. -F. Chak},
  journal={Biochemical and biophysical research communications},
  year={2003},
  volume={312 3},
  pages={708-15}
}
We have observed that the process of sporulation of the ispA-deficient mutant was delayed under phase-contrast microscopy. The protein profiles of the ispA-deficient mutant have been analyzed using two-dimensional gel electrophoresis. The results of a proteomic analysis using MALDI-TOF MS indicated that a sporulation-associated protein, pro- [Formula: see text], was upregulated, while two other sporulation-associated proteins, SpoVD and SpoVR, were downregulated in the ispA-deficient mutant. It… CONTINUE READING