The Invader assay for SNP genotyping.

  title={The Invader assay for SNP genotyping.},
  author={Michael Olivier},
  journal={Mutation research},
  volume={573 1-2},
The Invader assay uses a structure-specific flap endonuclease (FEN) to cleave a three-dimensional complex formed by hybridization of allele-specific overlapping oligonucleotides to target DNA containing a single nucleotide polymorphism (SNP) site. Annealing of the oligonucleotide complementary to the SNP allele in the target molecule triggers the cleavage of the oligonucleotide by cleavase, a thermostable FEN. Cleavage can be detected by several different approaches. Most commonly, the cleavage… CONTINUE READING


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