The Intracellular Fate of Salmonella Depends on the Recruitment of Kinesin

@article{Boucrot2005TheIF,
  title={The Intracellular Fate of Salmonella Depends on the Recruitment of Kinesin},
  author={Emmanuel Boucrot and Thomas Henry and Jean-Paul Borg and Jean Pierre Gorvel and St{\'e}phane M{\'e}resse},
  journal={Science},
  year={2005},
  volume={308},
  pages={1174 - 1178}
}
Salmonella enterica causes a variety of diseases, including gastroenteritis and typhoid fever. The success of this pathogen depends on its capacity to proliferate within host cells in a membrane-bound compartment. We found that the Salmonella-containing vacuole recruited the plus-end–directed motor kinesin. Bacterial effector proteins translocated into the host cell by a type III secretion system antagonistically regulated this event. Among these effectors, SifA targeted SKIP, a host protein… 

Analysis of kinesin accumulation on Salmonella-containing vacuoles.

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In vitro and in cellulo assays reveal a specific interaction between the two effectors and indicate that, contrary to what studies on infected cells suggested, interaction with PipB2 is sufficient to relieve the autoinhibition of kinesin-1.

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The Salmonella effector protein PipB2 is a linker for kinesin-1

The data indicate that the TTSS-2-mediated fine-tuning of kinesin-1 activity associated with the bacterial vacuole is crucial for the virulence of Salmonella.

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The role and significance of membrane compartment remodeling observed in infected cells and the bacterial and host cell pathways involved are focused on.

The Virulence Protein SopD2 Regulates Membrane Dynamics of Salmonella-Containing Vacuoles

SopD2 inhibits the vesicular transport and the formation of tubules that extend outward from the SCV and thereby contributes to the sifA− associated phenotypes and highlights the antagonistic roles played by SopD2 and SifA in the membrane dynamics of the vacuole.
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