The Internally Self-fertilizing Hermaphroditic Teleost Rivulus marmoratus (Cyprinodontiformes, Rivulidae) β-Actin Gene: Amplification and Sequence Analysis with Conserved Primers

@article{Lee2000TheIS,
  title={The Internally Self-fertilizing Hermaphroditic Teleost Rivulus marmoratus (Cyprinodontiformes, Rivulidae) β-Actin Gene: Amplification and Sequence Analysis with Conserved Primers},
  author={Jae-Seong Lee},
  journal={Marine Biotechnology},
  year={2000},
  volume={2},
  pages={161-166}
}
To determine the ease and feasibility of amplifying the β-actin gene in fish by the polymerase chain reaction (PCR), genomic DNAs of several fish (Rivulus, Southern top mouth minnow, common fat minnow, oily bitterling, carp, Far Eastern catfish, medaka, and European flounder) were extracted and used as a template with conserved primers, designed on the basis of high amino acid homology (approximately 98% or more). Among them, the self-fertilizing hermaphroditic fish Rivulus marmoratus was… CONTINUE READING

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